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The effect of hydroxyl radical-producing agents on the interaction of transferrin (Tf) with calf thymus double-stranded DNA (dsDNA) was investigated using fluorescence spectroscopy. Incubation of dsDNA with H2O2 and cumene hydroperoxide (CUOOH) resulted in an increase in fluorescence intensity (about 4-fold), which indicated a destabilization of the Tf-DNA interaction. Under the same experimental conditions, CUOOH and H2O2 cause a partial inhibition of Tf binding to poly(dG-dC).poly(dG-dC) (dG-dC). The CUOOH effect was stronger than the H2O2 effect. Cu(I) catalase added in excess was able to inhibit the fluorescence intensity increase produced by CUOOH. The results suggest that the intensity increase observed was due to hydroxyl radical formation resulting in the hydrolysis of dsDNA, which produced single-stranded DNA (ssDNA) fragments with a high negative charge density. A possible reaction mechanism for the generation of hydroxyl radicals by CUOOH is presented.
406 F.Supp.2d 904 (2005)
CITIGROUP, INC., and Citigroup Global Markets, Inc., Plaintiffs,
v.
Sedona VACUUM CLEANERS, INC., Defendant.
No. 05-CV-4298 (NGG).
United States District Court, E.D. New York.
December 16, 2005.
*905 Jennifer S. Ehrlich, J. David be359ba680
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